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and Bioassay Research
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+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-617.299.7367 (Int’l)
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sales@neoscientific.com
+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-888.733.6849
+1-617.299.7367 (Int’l)
| Reactivity | Human |
| Tested applications | WB IHC IF |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100 |
| Calculated MW | 107kDa |
| Observed MW | Refer to Figures |
| Immunogen | A synthetic peptide of human DDX58 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Synonym | DDX58;DKFZp434J1111;DKFZp686N19181;FLJ13599;RIG-I |
Western blot analysis of extracts of various cell lines, using DDX58 antibody.
Immunohistochemistry of paraffin-embedded human prostate using DDX58 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of MCF-7 cell using DDX58 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cell using DDX58 antibody. Blue: DAPI for nuclear staining.
cts viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling (4-7). Rig-I binds both dsRNA and viral ssRNA that contains a 5'-triphosphate end not seen in host RNA (8,9). Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses (10,11). The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-κB, TBK1/IKKε, and IRF-3/IRF-7 (12-15).
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