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+1-888.733.6849
+1-617.299.7367 (Int’l)
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+1-617.299.7367 (Int’l)
| Reactivity | Human Mouse |
| Tested applications | WB IHC IF |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Calculated MW | 43kDa |
| Observed MW | Refer to figures |
| Immunogen | Recombinant protein of human NELFE |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | j |
| Synonym | RD; RDP; RDBP; D6S45; NELF-E; |
Western blot analysis of extracts of various cell lines, using NELFE antibody.
Western blot analysis of extracts of various cell lines, using NELFE antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using NELFE antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using NELFE antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse Intestine using NELFE antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse liver using NELFE antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U20S cell using NELFE antibody.
The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
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