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sales@neoscientific.com
+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-888.733.6849
+1-617.299.7367 (Int’l)
| Reactivity | Human Mouse |
| Tested applications | WB IHC |
| Recommended Dilution | WB 1:1000 - 1:3000 IHC 1:50 - 1:100 |
| Calculated MW | 116kDa |
| Observed MW | Refer to Figures |
| Immunogen | Recombinant protein of human PARP1 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | defjp |
| Synonym | PARP; PARP1; PPOL; ADPRT; ADPRT1; PARP1-1; ADPRT 1; pADPRT-1; |
PARP1, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP1, the cleavage occurs between Asp214 and Gly215, which separates the PARP1 amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP1 helps cells to maintain their viability; cleavage of PARP1 facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
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